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BacTrack User Tutorial

BacTrack feature written and video tutorial: \

Tutorial

To start, either:

  • SETUP NEW EXPERIMENT

You must prepare, beforehand, three folders: red, green and blue (each containing image sequence of the appropriate channel).

  Images must be 8-bit PNGs, and must be named unanimously (having the same name, and increasing number at the end), e.g.:                                         
                                                                                                                                                             
      prpdesGFPmCherryWag05prop01_32_white0000.png                                                                                                           
      prpdesGFPmCherryWag05prop01_32_white0001.png                                                                                                           
      prpdesGFPmCherryWag05prop01_32_white0002.png                                                                                                           
      ...                                                                                                                                                    
  Don't worry, ImageJ will do this for you (by deafault), if you say File->Save As->Image sequence.
  Also, note that once you've processed the movie (i.e. performed cell segmentations) you can measure on a whole different set of images (typically original TIFFs), as long as the ROIs (cell selections) hit the same spot.                                                           
  These folders should not be moved afterwards (in this version) - the program will expect images to be at the same place when you (later) load the experiment.
  Should you move them, you'll have to edit the experiment properties and set the new file locations.
  In other words, images WILL NOT be saved to database, only the calculated/defined ROIs, measurements etc.                                                  
                                                                                                                                                             

* LOAD THE EXISTING EXPERIMENT

  (beware of the last sentence, just above) 

Video Tutorial

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