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Welcome to BactImAs Wiki pages

BactImAs is an open-source multi platform java application intended to assist the researcher in tracking various organisms in sequences of images and obtaining and visualizing their quantitative data.

Bactimas paper is published here: [[1]]

Motivation: A common task in today’s synthetic and systems biology studies is the analysis of various features of organisms using movies obtained through time-lapse microscopy. \\ This process consists of identifying organisms on a large number of images and measuring their features whilst keeping track of their lineage which is **a very cumbersome and error prone task** for the researcher. To facilitate the analysis, various mostly automated tools and algorithms were developed in recent years but all of them targeting rather specific organisms and/or laboratory setups, since a general purpose automated solution to this detection problem is extremely hard if not impossible.

Solution: We've designed BactImAs as a "platform", that is as a collection of somewhat independent well defined modules working together. Such setup facilitates maintenance, customization and upgrades. This property is particularly useful for the tracking algorithms - in BactImAs it is possible to include multiple tracking algorithms ("best-of-breed approach") and thus use the best fitted algoritm for the problem at hand. \\ It is even possible to mix-and-match algorithms in a way to segment one part of the movie with algorithm A and the other part with algorithm B.

BactImAs uses a semi-automated approach: it relies on the user to define the initial cells and all cell divisions. Other than that, the application tracks cells using a our newly developed algorithm. At any point, the user can intervene and correct any unsatisfactory cell detections.

BactImAs provides the following features:

 * Easy to use GUI interface 
 * Frame anlignment algorithm 
 * Novel mycobacteria segmentation algorithm
 * Novel configurable lineage tree visualization
 * Automated acquisition of quantitative date
 * Relational database storage, SQL interface to data
 * Data export (CSV)

Example reports produced by BactImAs:

  • BW Report video generated from the program (black outline - computer, white - human

  • Color report video generated from the program (green outline - human, red - human)